Safety of 100 mg NADH

Animal studies demonstrating a large safety margin:

26-week oral rat toxicity, equivalent to ~175 mg/day human dose:

https://pubmed.ncbi.nlm.nih.gov/12635493/

Beagle dog toxicity study, including oral 20–150 mg/kg and IV escalation up to 1000 mg/kg:

*https://pubmed.ncbi.nlm.nih.gov/15312041/*

Synthesis & Takeaways

Human clinical experience at 10–20 mg/day with no serious adverse events:

Forsyth et al., NADH in Chronic Fatigue Syndrome (10 mg/day)

*https://pubmed.ncbi.nlm.nih.gov/10071523/*

Demarin et al., NADH in Alzheimer’s Disease (10 mg/day)

*https://pubmed.ncbi.nlm.nih.gov/15134388/*

Castro‑Marrero et al., NADH + CoQ10 in CFS (20 mg/day)

*https://pubmed.ncbi.nlm.nih.gov/25386668/*

Vitamin B3 regulatory perspective:

100 mg NADH ≈ 18.4 mg niacinamide – well under EU’s upper limit of 35 mg/day.

Efficacy at 10–20 mg/day

Chronic Fatigue Syndrome:

Forsyth et al. 1999 showed 31 % response in CFS vs 8 % placebo at 10 mg/day

PubMed: